diff --git a/src/UI/Timeline/AnimationTimeline.gd b/src/UI/Timeline/AnimationTimeline.gd
index e1c1cd0e4..685f7e1f9 100644
--- a/src/UI/Timeline/AnimationTimeline.gd
+++ b/src/UI/Timeline/AnimationTimeline.gd
@@ -1,7 +1,14 @@
 extends Panel
 
+## Emitted when the animation starts playing.
 signal animation_started(forward: bool)
+## Emitted when the animation reaches the final frame and is not looping,
+## or if the animation is manually paused.
+## Note: This signal is not emitted if the animation is looping.
 signal animation_finished
+## Emitted when the animation loops, meaning when it reaches the final frame
+## and the animation keeps playing.
+signal animation_looped
 
 enum LoopType { NO, CYCLE, PINGPONG }
 
@@ -688,9 +695,11 @@ func _on_AnimationTimer_timeout() -> void:
 					animation_timer.wait_time = (
 						project.frames[project.current_frame].duration * (1 / fps)
 					)
+					animation_looped.emit()
 					animation_timer.start()
 				LoopType.PINGPONG:
 					animation_forward = false
+					animation_looped.emit()
 					_on_AnimationTimer_timeout()
 
 	else:
@@ -713,9 +722,11 @@ func _on_AnimationTimer_timeout() -> void:
 					animation_timer.wait_time = (
 						project.frames[project.current_frame].duration * (1 / fps)
 					)
+					animation_looped.emit()
 					animation_timer.start()
 				LoopType.PINGPONG:
 					animation_forward = true
+					animation_looped.emit()
 					_on_AnimationTimer_timeout()
 	frame_scroll_container.ensure_control_visible(
 		Global.frame_hbox.get_child(project.current_frame)
diff --git a/src/UI/Timeline/LayerButton.gd b/src/UI/Timeline/LayerButton.gd
index 8ef90e984..a82cceb51 100644
--- a/src/UI/Timeline/LayerButton.gd
+++ b/src/UI/Timeline/LayerButton.gd
@@ -47,6 +47,7 @@ func _ready() -> void:
 		layer.audio_changed.connect(func(): audio_player.stream = layer.audio)
 		add_child(audio_player)
 		Global.animation_timeline.animation_started.connect(_on_animation_started)
+		Global.animation_timeline.animation_looped.connect(_on_animation_looped)
 		Global.animation_timeline.animation_finished.connect(_on_animation_finished)
 	custom_minimum_size.y = Global.animation_timeline.cel_size
 	label.text = layer.name
@@ -78,6 +79,14 @@ func _on_animation_started(_dir: bool) -> void:
 	_play_audio()
 
 
+func _on_animation_looped() -> void:
+	var layer := Global.current_project.layers[layer_index]
+	if layer is AudioLayer:
+		if layer.playback_frame != 0:
+			if is_instance_valid(audio_player):
+				audio_player.stop()
+
+
 func _on_animation_finished() -> void:
 	animation_running = false
 	if is_instance_valid(audio_player):